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* Corresponding author 1 Caractérisation du tissu osseux par imagerie : techniques et applications 2 Alcoolisation précoce et vulnerabilité à la dépendance 3 http:-www.u-picardie.fr-decouverte-sante-pagesliees-grap-accueilgrap.html Alcoolisation précoce et vulnerabilité à la dépendance

Abstract : BACKGROUND: Different models are used to study the effects of chronic alcohol consumption on bone tissue in the rat. However, the current models take several months to show indices of osteopenia as observed in chronic drinkers. Numerous studies have supported that chronic and intermittent exposure to ethanol vapors has predictive validity as a model of alcohol dependence in humans. However, this model has never been applied to bone research to study its effects on the parameters that define osteopenia. This was the goal of this study in the rat. METHODS: Male Wistar rats were exposed to ethanol vapor inhalation n = 6 or air controls, n = 6. Animals were exposed to chronic 11 weeks and intermittent 14 hours a day ethanol vapor reaching stable blood alcohol levels BALs; 150 to 250 mg-dl at the end of the third week of inhalation. After the sacrifice, right and left femur and tibia were dissected free of fat and connective tissue and bone mineral density BMD was assessed by dual X-ray absorptiometry. The microarchitecture of the femur was studied using microcomputed tomography. RESULTS: The BMD of the left and right femurs and the left tibia was lower in the ethanol group compared with the control group. The bone volume fraction BV-TV and the bone surface density BS-TV were lower in the ethanol group compared with control animals. The trabecular number Tb.N was lower in the ethanol group while the trabecular spacing was higher. CONCLUSIONS: The decrease in the BMD, BV-TV, and Tb.N is in the same range as what is observed in human drinkers and what is reported with other animal alcohol models Lieber-DeCarli liquid diet, ethanol in the tap water. Therefore, this model could be useful to study the effects of chronic alcohol consumption in the bone research field and has the advantage of controlling easily targeted BALs.

Mots-clés : alcoolisme alcool inhalation rat ostéoporose ostéopénie addiction





Autor: Delphine Maurel - Christelle Jaffré - Emmanuelle Simon O-Brien - Carine Tournier - Hakim Houchi - Claude-Laurent Benhamou - Mick

Fuente: https://hal.archives-ouvertes.fr/



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