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BioMed Research International - Volume 2016 2016, Article ID 4302754, 9 pages -

Research Article

Department of Andrology and Sexology, Faculty of Medicine, Cairo University, Cairo, Egypt

Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt

Department of Andrology and Sexology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt

Department of Andrology and Sexology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Received 9 August 2016; Accepted 16 November 2016

Academic Editor: Robert Stoehr

Copyright © 2016 Taymour Mostafa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. This study aimed to assess seminal miRNA relationship with seminal apoptotic markers and oxidative stress OS in infertile men associated with varicocele Vx. Methods. In all, 220 subjects were divided into the following groups: fertile normozoospermic men, fertile normozoospermic men with Vx, infertile oligoasthenoteratozoospermic OAT men without Vx, and infertile OAT men with Vx. They were subjected to history taking, clinical examination, and semen analysis. In their semen, the following were estimated: miRNA-122, miRNA-181a, and miRNA-34c5 using quantitative real-time PCR, apoptotic markers BAX, BCL2 protein expression, and OS markers malondialdehyde MDA and glutathione peroxidase GPx. Results. The mean levels of seminal miRNA-122, miRNA-181a, and miRNA-34c5 were significantly reduced in infertile OAT men with Vx compared with other groups coupled with Vx grade and Vx bilaterality. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 were positively correlated with sperm concentration, total sperm motility, sperm normal morphology, seminal GPx, and seminal BCL2 and negatively correlated with seminal MDA and seminal BAX. Conclusions. Seminal miRNA-122, miRNA-181a, and miRNA-34c5 are decreased in infertile OAT men with Vx associated with increased Vx grade and Vx bilaterality. In addition, they are positively correlated with sperm parameters and negatively correlated with OS, apoptotic markers.





Autor: Taymour Mostafa, Laila A. Rashed, Nashaat I. Nabil, Ihab Osman, Rashad Mostafa, and Mohamed Farag

Fuente: https://www.hindawi.com/



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