In Vitro Assessment of Cytochrome P450 2C19 Potential of NaoxintongReportar como inadecuado

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Evidence-Based Complementary and Alternative MedicineVolume 2012 2012, Article ID 430262, 6 pages

Research Article

Department of Internal Medicine, Fujian Provincial Cardiovascular Disease Institute, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350001, China

Clinical Discipline of Chinese and Western Integrative Medicine, Fujian University on Traditional Chinese Medicine, Fuzhou 350108, China

Received 1 July 2011; Revised 22 October 2011; Accepted 8 November 2011

Academic Editor: Keji Chen

Copyright © 2012 Hui Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effects of Buchang Naoxintong Capsules BNCs on S-mephenytoin 4′-hydroxylation activities in human liver microsomes in vitro were assessed. Human liver microsome was prepared by different ultracentrifugation. Human liver microsome incubation experiment was carried out to assay BNC on S-mephenytoin 4′-hydroxylation activities. The 4′-hydroxylation of S-mephenytoin, a representative substrate toward CYP2C19, was increased by phenytoin sodium positive control. After the incubation, the metabolites of the substrates 4′-OH-mephenytoin were determined by HPLC. Results showed that both phenytoin sodium and BNC showed obvious increase effect on CYP2C19. The enzymatic reaction of BNC was observed with concentrations ranging from 5 μg-mL to 250 μg-mL. Compared to blank, the increase effect of BNC showed significant difference from the beginning of concentration of 150 μg-mL . The conclusion was that BNC showed obvious increase effect on the catalytic activities of drug-metabolising CYP2C19 enzyme.

Autor: Hui Chen, Ya Zhang, Xiaoying Wu, Candong Li, and Huan Wang



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