High In Vitro Antibacterial Activity of Pac-525 against Porphyromonas gingivalis Biofilms Cultured on TitaniumReportar como inadecuado

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BioMed Research International - Volume 2015 2015, Article ID 909870, 8 pages -

Research Article

Department of Prosthodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China

Department of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China

Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China

Received 2 July 2014; Accepted 3 January 2015

Academic Editor: Henny C. van der Mei

Copyright © 2015 Ji-yin Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In order to investigate the potential of short antimicrobial peptides AMPs as alternative antibacterial agents during the treatment of peri-implantitis, the cytotoxic activity of three short AMPs, that is, Pac-525, KSL-W, and KSL, was determined using the MTT assay. The antimicrobial activity of these AMPs, ranging in concentration from 0.0039 mg-mL to 0.5 mg-mL, against the predominant planktonic pathogens, including Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis, involved in peri-implantitis was investigated. Furthermore, 2-day-old P. gingivalis biofilms cultured on titanium surfaces were treated with Pac-525 and subsequently observed and analysed using confocal laser scanning microscopy CLSM. The average cell proliferation curve indicated that there was no cytotoxicity due to the three short AMPs. The minimum inhibitory concentration and minimum bactericidal concentration values of Pac-525 were 0.0625 mg-mL and 0.125 mg-mL, respectively, for P. gingivalis and 0.0078 mg-mL and 0.0156 mg-mL, respectively, for F. nucleatum. Using CLSM, we confirmed that compared to 0.1% chlorhexidine, 0.5 mg-mL of Pac-525 caused a significant decrease in biofilm thickness and a decline in the percentage of live bacteria. These data indicate that Pac-525 has unique properties that might make it suitable for the inhibition the growth of pathogenic bacteria around dental implants.

Autor: Ji-yin Li, Xue-jin Wang, Li-na Wang, Xiao-xia Ying, Xiang Ren, Hui-ying Liu, Li Xu, and Guo-wu Ma

Fuente: https://www.hindawi.com/


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