Antitumor Activities of Ethyl Acetate Extracts from Selaginella doederleinii Hieron In Vitro and In Vivo and Its Possible MechanismReport as inadecuate




Antitumor Activities of Ethyl Acetate Extracts from Selaginella doederleinii Hieron In Vitro and In Vivo and Its Possible Mechanism - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative Medicine - Volume 2015 2015, Article ID 865714, 9 pages -

Research ArticleHubei University of Traditional Chinese Medicine, Key Laboratory of TCM Resource and TCM Compound Co-Constructed by Hubei Province and Ministry of Education, Wuhan 430065, China

Received 28 July 2014; Accepted 30 November 2014

Academic Editor: Senthamil R. Selvan

Copyright © 2015 Jia-zhi Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antitumor activities of ethyl acetate extracts from Selaginella doederleinii Hieron SD extracts in vitro and in vivo and its possible mechanism were investigated. HPLC method was developed for chemical analysis. SD extracts were submitted to 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide MTT assay on different cells, flow cytometry, and RT-PCR analysis using HepG2 cell and antitumor activity in vivo using H-22 xenograft tumor mice. Six biflavonoids from SD extracts were submitted to molecular docking assay. The results showed that SD extracts had considerable antitumor activity in vitro and in vivo without obvious toxicity on normal cells and could induce cell apoptosis. The mechanisms of tumorigenesis and cell apoptosis induced by SD extracts may be associated with decreasing the ratio of bcl-2 and bax mRNA level, activating caspase-3, suppressing survivin, and decreasing the gene expression of COX-2, 5-LOX, FLAP, and 12-LOX mRNA. The main active component in SD extracts is biflavonoids and some exhibited strong interactions with COX-2, 5-LOX, 12-LOX, and 15-LOX. These results offering evidence of possible mechanisms of SD extracts suppress cell proliferation and promote apoptosis and provide the molecular theoretical basis of clinical application of S. doederleinii for cancer therapy.





Author: Jia-zhi Wang, Juan Li, Ping Zhao, Wen-tao Ma, Xie-he Feng, and Ke-li Chen

Source: https://www.hindawi.com/



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