Prevention of unloading-induced atrophy by vitamin E supplementation: Links between oxidative stress and soleus muscle proteolysisReportar como inadecuado




Prevention of unloading-induced atrophy by vitamin E supplementation: Links between oxidative stress and soleus muscle proteolysis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 PICM - Physiologie intégrative, cellulaire et moléculaire 2 Bioénergétique fondamentale et appliquée

Abstract : Exposure to reduced activity induces skeletal muscle atrophy. Oxidative stress might contribute to muscle wasting via proteolysis activation. This study aimed to test two hypotheses in rats. First, supplementation of the antioxidant vitamin E, prior and during the phase of unloading, would partly counteract unloading-induced soleus muscle atrophy. Secondly, vitamin E supplementation would decrease the rate of muscle proteolysis by reducing expression of calpains, caspases-3 -9, and -12, and E3 ubiquitin ligases MuRF1 and MAFbx. Soleus muscle atrophy -49% induced by 14 days of hindlimb unloading was reduced to only 32% under vitamin E. Vitamin E partly prevented the decrease in type I and IIa fiber size. Supplementation increased HSP72 content and suppressed the rise in muscle level of thiobarbituric acid-reactive substance caused by unloading but failed to modify the lower ratio of reduced vs oxidized glutathione, the higher uncoupling proteins mRNA, and the antioxidant enzyme activities superoxide dismutase, catalase, and glutathione peroxidase observed after unloading. Vitamin E treatment abolished the large upregulation of caspases-9 and -12 and MuRF1 transcripts in unloaded muscle and greatly decreased the upregulation of mu-calpain, caspase-3, and MAFbx mRNA. In conclusion, the protective effect of vitamin E might be due to modulation of muscle proteolysis-related genes rather than to its antioxidant function.

Keywords : Muscle atrophy Vitamin E Oxidative stress Uncoupling proteins Heat shock protein Caspases Ubiquitine ligases





Autor: Stéphane Servais - Dominique Letexier - Roland Favier - Claude Duchamp - Dominique Desplanches -

Fuente: https://hal.archives-ouvertes.fr/



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