Biochemical Characterization, Thermal Stability, and Partial Sequence of a Novel Exo-Polygalacturonase from the Thermophilic Fungus Rhizomucor pusillus A13.36 Obtained by Submerged CultivationReportar como inadecuado




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BioMed Research International - Volume 2016 2016, Article ID 8653583, 10 pages -

Research Article

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista UNESP, São José do Rio Preto, SP, Brazil

Universidade de São Paulo USP, FFCLRP, Ribeirão Preto, SP, Brazil

Universidade Estadual de Campinas UNICAMP, Campinas, SP, Brazil

Received 10 July 2016; Revised 26 October 2016; Accepted 7 November 2016

Academic Editor: Muhammad I. Rajoka

Copyright © 2016 Lucas Vinícius Trindade et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work reports the production of an exo-polygalacturonase exo-PG by Rhizomucor pusillus A13.36 in submerged cultivation SmC in a shaker at 45°C for 96 h. A single pectinase was found and purified in order to analyze its thermal stability, by salt precipitation and hydrophobic interaction chromatography. The pectinase has an estimated Mw of approximately 43.5–47 kDa and optimum pH of 4.0 but is stable in pH ranging from 3.5 to 9.5 and has an optimum temperature of 61°C. It presents thermal stability between 30 and 60°C, has 70% activation in the presence of Ca

, and was tested using citrus pectin with a degree of methyl esterification DE of 26%. for irreversible denaturation was 125.5 kJ-mol with positive variations of entropy and enthalpy for that and values were around 50 kJ-mol. The hydrolysis of polygalacturonate was analyzed by capillary electrophoresis which displayed a pattern of sequential hydrolysis exo. The partial identification of the primary sequence was done by MS MALDI-TOF and a comparison with data banks showed the highest identity of the sequenced fragments of exo-PG from R. pusillus with an exo-pectinase from Aspergillus fumigatus. Pectin hydrolysis showed a sigmoidal curve for the Michaelis-Menten plot.





Autor: Lucas Vinícius Trindade, Carla Desagiacomo, Maria de Lourdes Teixeira de Moraes Polizeli, André Ricardo de Lima Damasio, Ali

Fuente: https://www.hindawi.com/



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