Alterations in Lipid Mediated Signaling and Wnt-β-Catenin Signaling in DMH Induced Colon Cancer on Supplementation of Fish OilReportar como inadecuado




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BioMed Research InternationalVolume 2014 2014, Article ID 832025, 11 pages

Research Article

Department of Biochemistry, Panjab University, Chandigarh 160014, India

Department of Histopathology, Post Graduate Institute of Medical Education and Research PGIMER, Chandigarh 160012, India

Received 19 February 2014; Revised 8 April 2014; Accepted 21 April 2014; Published 5 June 2014

Academic Editor: Saulius Butenas

Copyright © 2014 Shevali Kansal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ceramide mediates inhibition of cyclooxygenase-2 COX-2 which catalyzes formation of prostaglandin further activating peroxisome proliferator-activated receptorγ PPARγ and Wnt-β-catenin pathway; and hence plays a critical role in cancer. Therefore, in current study, ceramide, COX-2, 15-deoxy prostaglandin J215-deoxy PGJ2, PPARγ, and β-catenin were estimated to evaluate the effect of fish oil on lipid mediated and Wnt-β-catenin signaling in colon carcinoma. Male Wistar rats in Group I received purified diet while Groups II and III received modified diet supplemented with FO : CO1 : 1 and FO : CO2.5 : 1, respectively. These were further subdivided into controls receiving ethylenediaminetetraacetic acid and treated groups receiving dimethylhydrazine dihydrochloride DMH-week for 4 weeks. Animals sacrificed 48 hours after last injection constituted initiation phase and those sacrificed after 16 weeks constituted postinitiation phase. Decreased ceramide and increased PPARγ were observed in postinitiation phase only. On receiving FO+CO1 : 1+DMH and FO+CO2.5 : 1+DMH in both phases, ceramide was augmented whereas COX-2, 15-deoxy PGJ2, and nuclear translocation of β-catenin were reduced with respect to cancerous animals. Decrease was more significant in postinitiation phase with FO+CO2.5 : 1+DMH. Treatment with oils increased PPARγ in initiation phase but decreased it in postinitiation phase. Hence, fish oil altered lipid mediated signalling in a dose and time dependent manner so as to inhibit progression of colon cancer.





Autor: Shevali Kansal, Kim Vaiphei, and Navneet Agnihotri

Fuente: https://www.hindawi.com/



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