Cytoskeletal Strains in Modeled Optohydrodynamically Stressed Healthy and Diseased Biological CellsReportar como inadecuado

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Journal of BiophysicsVolume 2012 2012, Article ID 830741, 7 pages

Research Article

Regenerative Bioengineering Laboratory, Department of Biology, Science Research and Teaching Center SRTC, Portland State University, P.O. Box 751, Portland, OR 97207, USA

Department of Surgery, Oregon Health and Science University, Portland, OR 97239, USA

Center for Allaying Health Disparities through Research and Education CADRE and Department of Mathematics and Computer Science, Central State University, Wilberforce, OH 45384, USA

Received 27 September 2012; Revised 6 November 2012; Accepted 6 November 2012

Academic Editor: George Perry

Copyright © 2012 Sean S. Kohles et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Controlled external chemomechanical stimuli have been shown to influence cellular and tissue regeneration-degeneration, especially with regards to distinct disease sequelae or health maintenance. Recently, a unique three-dimensional stress state was mathematically derived to describe the experimental stresses applied to isolated living cells suspended in an optohydrodynamic trap optical tweezers combined with microfluidics. These formulae were previously developed in two and three dimensions from the fundamental equations describing creeping flows past a suspended sphere. The objective of the current study is to determine the full-field cellular strain response due to the applied three-dimensional stress environment through a multiphysics computational simulation. In this investigation, the multiscale cytoskeletal structures are modeled as homogeneous, isotropic, and linearly elastic. The resulting computational biophysics can be directly compared with experimental strain measurements, other modeling interpretations of cellular mechanics including the liquid drop theory, and biokinetic models of biomolecule dynamics. The described multiphysics computational framework will facilitate more realistic cytoskeletal model interpretations, whose intracellular structures can be distinctly defined, including the cellular membrane substructures, nucleus, and organelles.

Autor: Sean S. Kohles, Yu Liang, and Asit K. Saha



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