Single QTL mapping and nucleotide-level resolution of a physiologic trait in wine Saccharomyces cerevisiae strains.Reportar como inadecuado




Single QTL mapping and nucleotide-level resolution of a physiologic trait in wine Saccharomyces cerevisiae strains. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 Faculté d-Oenologie Bordeaux II 2 Unité de Microbiologie et Génétique 3 MAGNOME - Models and Algorithms for the Genome INRIA Futurs, Université Sciences et Technologies - Bordeaux 1, École Nationale Supérieure d-Électronique, Informatique et Radiocommunications de Bordeaux ENSEIRB, CNRS - Centre National de la Recherche Scientifique : UMR5800 4 BMC - Laboratoire de biologie moléculaire de la cellule

Abstract : Natural Saccharomyces cerevisiae yeast strains exhibit very large genotypic and phenotypic diversity. However, the link between phenotype variation and genetic determinism is still difficult to identify, especially in wild populations. Using genome hybridization on DNA microarrays, it is now possible to identify single-feature polymorphisms among divergent yeast strains. This tool offers the possibility of applying quantitative genetics to wild yeast strains. In this instance, we studied the genetic basis for variations in acetic acid production using progeny derived from two strains from grape must isolates. The trait was quantified during alcoholic fermentation of the two strains and 108 segregants derived from their crossing. A genetic map of 2212 markers was generated using oligonucleotide microarrays, and a major quantitative trait locus QTL was mapped with high significance. Further investigations showed that this QTL was due to a nonsynonymous single-nucleotide polymorphism that targeted the catalytic core of asparaginase type I ASP1 and abolished its activity. This QTL was only effective when asparagine was used as a major nitrogen source. Our results link nitrogen assimilation and CO2 production rate to acetic acid production, as well as, on a broader scale, illustrating the specific problem of quantitative genetics when working with nonlaboratory microorganisms.

Keywords : acetic acid QTL mapping DNA microarray aneuploidy ASP1 wine fermentation





Autor: Philippe Marullo - Michel Aigle - Marina Bely - Isabelle Masneuf-Pomarede - Pascal Durrens - Denis Dubourdieu - Gaël Yvert -

Fuente: https://hal.archives-ouvertes.fr/



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