A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose TissueReportar como inadecuado




A Model of Insulin Resistance in Mice, Born to Diabetic Pregnancy, Is Associated with Alterations of Transcription-Related Genes in Pancreas and Epididymal Adipose Tissue - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of ObesityVolume 2011 2011, Article ID 654967, 11 pages

Research Article

Faculty of Life Sciences, University of Bourgogne, UPRES EA 4183 Lipides et Signalisation Cellulaire, 6 Boulevard Gabriel, 21000 Dijon, France

Laboratory of Cell Biology and Physiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi and Institute of Biomedical and Applied Sciences ISBA, 01 BP 918 Cotonou, Benin

Centre for Integrative Genomics, University of Lausanne, Bâtiment Génopode, 5è Etage, 1015 Lausanne, Switzerland

Received 7 June 2010; Accepted 30 August 2010

Academic Editor: Francesco Saverio Papadia

Copyright © 2011 Akadiri Yessoufou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. This study is conducted on a model of insulin-resistant IR mice born to dams which were rendered diabetic by the administration of streptozotocin. Methods. Adult IR and control offspring were selected and we determined the mRNA expression of transcription factors known to modulate pancreatic and adipose tissue activities and inflammation. Results. We observed that serum insulin increased, and the mRNA of insulin gene transcription factors, Pdx-1, Nkx6.1 and Maf-A, were upregulated in IR mice pancreas. Besides, their pancreatic functional capacity seemed to be exhausted as evidenced by low expression of pancreatic Glut2 and glucokinase mRNA. Though IR offspring exhibited reduced epididymal adipose tissue, their adipocytes seemed to be differentiated into macrophage-like cells, as they exhibited upregulated CD14 and CD68 antigens, generally expressed by macrophages. However, there was no peripheral macrophages infiltration into epididymal adipose tissue, as the expression of F4-80, a true macrophage marker, was undetectable. Furthermore, the expression of IL-6, TNF-α and TLR-2, key players of insulin resistance, was upregulated in the adipose tissue of IR offspring. Conclusion. Insulin resistant state in mice, born to diabetic pregnancy, alters the expression of function-related genes in pancreas and epididymal adipose tissue and these offspring are prone to develop metabolic syndrome.





Autor: Akadiri Yessoufou, Kabirou Moutairou, and Naim Akhtar Khan

Fuente: https://www.hindawi.com/



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