Inhibition of JAK-STAT ERK-MAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem CellsReportar como inadecuado

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Stem Cells International - Volume 2016 2016, Article ID 5127984, 11 pages -

Research Article

CSIRO Health and Biosecurity, East Geelong, VIC 3219, Australia

Monash Institute for Medical Research, Monash University, Clayton, VIC 3168, Australia

Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia

Department of Materials Engineering, Monash Institute of Medical Engineering, Faculty of Engineering, Monash University, Clayton, VIC 3168, Australia

South Australian Research and Development Institute SARDI, Turretfield Research Centre, Rosedale, SA 5350, Australia

Received 13 July 2015; Revised 25 October 2015; Accepted 29 October 2015

Academic Editor: Tao-Sheng Li

Copyright © 2016 Luis F. Malaver-Ortega et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pluripotent stem cells PSCs fall in two states, one highly undifferentiated, the naïve state, and the primed state, characterized by the inability to contribute to germinal lineage. Several reports have demonstrated that these states can be modified by changes to the cell culture conditions. With the advent of nuclear reprogramming, bovine induced pluripotent stem cells biPSCs have been generated. These cells represent examples of a transient-intermediate state of pluripotency with remarkable characteristics and biotechnological potential. Herein, we generated and characterized biPSC. Next, we evaluated different culture conditions for the ability to affect the expression of the set of core pluripotent transcription factors in biPSC. It was found that the use of 6-bromoindirubin-3-oxime and Sc1 inhibitors alone or in combination with 5-AzaC induced significantly higher levels of expression of endogenous REX1, OCT4, NANOG, and SOX2. Furthermore, LIF increased the levels of expression of OCT4 and REX1, compared with those cultured with LIF + bFGF. By contrast, bFGF decreased the levels of expression for both REX1 and OCT4. These results demonstrate that the biPSC gene expression profile is malleable by modification of the cell culture conditions well after nuclear reprogramming, and the culture conditions may determine their differentiation potential.

Autor: Luis F. Malaver-Ortega, Huseyin Sumer, Jun Liu, and Paul J. Verma



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