Optimization of pressurized liquid extraction using a multivariate chemometric approach and comparison of solid phase extraction cleanup steps for the determination of polycyclic aromatic hydrocarbons in mossesReportar como inadecuado




Optimization of pressurized liquid extraction using a multivariate chemometric approach and comparison of solid phase extraction cleanup steps for the determination of polycyclic aromatic hydrocarbons in mosses - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1 LCA - Laboratoire de Chimie Agro-Industrielle

Abstract : A factorial design was used to optimize the extraction of polycyclic aromatic hydrocarbons PAHs from mosses, plants used as biomonitors of air pollution. The analytical procedure consists of pressurized liquid extraction PLE followed by solid-phase extraction SPE cleanup, in association with analysis by high performance liquid chromatography coupled with fluorescence detection HPLC-FLD. For method development, homogeneous samples were prepared with large quantities of the mosses Isothecium myosuroides Brid. and Hypnum cupressiforme Hedw., collected from a Spanish Nature Reserve. A factorial design was used to identify the optimal PLE operational conditions: 2 static cycles of 5 min at 80°C. The analytical procedure performed with PLE showed similar recoveries ∼70% and total PAH concentrations ∼200 ng g-1 as found using Soxtec extraction, with the advantage of reducing solvent consumption by 3 30 mL against 100 mL per sample, and taking a fifth of the time 24 samples extracted automatically in 8 h against 2 samples in 3.5 h. The performance of SPE normal phases NH2, Florisil®, silica and activated aluminium generally used for organic matrix cleanup was also compared. Florisil® appeared to be the most selective phase and ensured the highest PAH recoveries. The optimal analytical procedure was validated with a reference material and applied to moss samples from a remote Spanish site in order to determine spatial and inter-species variability.

Keywords : Polycyclic aromatic hydrocarbon PAH Moss Pressurized liquid extraction Solid phase extraction High performance liquid chromatography Biomonitoring





Autor: Louise Foan - Valérie Simon -

Fuente: https://hal.archives-ouvertes.fr/



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