What Does Low-Intensity rTMS Do to the CerebellumReportar como inadecuado

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* Corresponding author 1 B2A - Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing 2 School of Animal Biology 3 Institut de la longévité

Abstract : Non-invasive stimulation of the human cerebellum, such as by transcranial magnetic stimulation TMS, is increasingly used to investigate cerebellar function and identify potential treatment for cerebellar dysfunction. However, the effects of TMS on cerebellar neurons remain poorly defined. We applied low-intensity repetitive TMS LI-rTMS to the mouse cerebellum in vivo and in vitro and examined the cellular and molecular sequelae. In normal C57-Bl6 mice, 4 weeks of LI-rTMS using a complex biomimetic high-frequency stimulation BHFS alters Purkinje cell PC dendritic and spine morphology; the effects persist 4 weeks after the end of stimulation. We then evaluated whether LI-rTMS could induce climbing fibre CF reinnervation to denervated PCs. After unilateral pedunculotomy in adult mice and 2 weeks sham or BHFS stimulation, VGLUT2 immunohistochemistry was used to quantify CF reinnervation. In contrast to sham, LI-rTMS induced CF reinnervation to the denervated hemicerebellum. To examine potential mechanisms underlying the LI-rTMS effect, we verified that BHFS could induce CF reinnervation using our in vitro olivocerebellar explants in which denervated cerebellar tissue is co-cultured adjacent to intact cerebella and treated with brain-derived neurotrophic factor BDNF as a positive control, sham or LI-rTMS for 2 weeks. Compared with sham, BDNF and BHFS LI-rTMS significantly increased CF reinnervation, without additive effect. To identify potential underlying mechanisms, we examined intracellular calcium flux during the 10-min stimulation. Complex high-frequency stimulation increased intracellular calcium by release from intracellular stores. Thus, even at low intensity, rTMS modifies PC structure and induces CF reinnervation.

Keywords : climbing fibres magnetic stimulation Purkinje cell spatial learning reinnervation rTMS

Autor: N Morellini - S Grehl - A Tang - J Rodger - J Mariani - Am Lohof - Rachel M Sherrard -

Fuente: https://hal.archives-ouvertes.fr/


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