Expression of ECM proteins fibulin-1 and -2 in acute and chronic liver disease and in cultured rat liver cellsReportar como inadecuado

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Cell and Tissue Research

, Volume 337, Issue 3, pp 449–462

First Online: 17 July 2009Received: 23 January 2009Accepted: 25 May 2009


Fibulin-2 has previously been considered as a marker to distinguish rat liver myofibroblasts from hepatic stellate cells. The function of other fibulins in acute or chronic liver damage has not yet been investigated. The aim of this study has been to evaluate the expression of fibulin-1 and -2 in models of rat liver injury and in human liver cirrhosis. Their cellular sources have also been investigated. In normal rat liver, fibulin-1 and -2 were both mainly present in the portal field. Fibulin-1-coding transcripts were detected in total RNA of normal rat liver, whereas fibulin-2 mRNA was only detected by sensitive, real-time quantitative polymerase chain reaction. In acute liver injury, the expression of fibulin-1 was significantly increased 17.23-fold after 48 h, whereas that of fibulin-2 was not modified. The expression of both fibulin-1 and -2 was increased in experimental rat liver cirrhosis 19.16- and 26.47-fold, respectively. At the cellular level, fibulin-1 was detectable in hepatocytes -activated- hepatic stellate cells, and liver myofibroblasts 2.71-, 122.65-, and 469.48-fold over the expression in normal rat liver, whereas fibulin-2 was restricted to liver myofibroblasts and was regulated by transforming growth factor beta-1 TGF-β1 in 2-day-old hepatocyte cultures and in liver myofibroblasts. Thus, fibulin-1 and -2 respond differentially to single and repeated damaging noxae, and their expression is differently present in liver cells. Expression of the fibulin-2 gene is regulated by TGF-β1 in liver myofibroblasts.

KeywordsCarbon tetrachloride Extracellular matrix Hepatic stellate cells Liver cirrhosis Liver myofibroblasts Rat Wistar Human The work was supported by SFB 402 funding.

Fabio Piscaglia and József Dudás contributed equally to this work.

Rupert Timpl died in 2003.

This article is dedicated to Professor Karl-Hermann Meyer zu Büschenfelde on the occasion of his 80th birthday.

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Autor: Fabio Piscaglia - József Dudás - Thomas Knittel - Paola Di Rocco - Dominik Kobold - Bernhard Saile - Maria Assunta Zocc


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