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BMC Medical Genetics

, 10:89

First Online: 14 September 2009Received: 18 May 2009Accepted: 14 September 2009

Abstract

BackgroundIt is estimated that 10-15% of all clinically recognised pregnancies result in a spontaneous abortion or miscarriage. Previous studies have indicated that in up to 50% of first trimester miscarriages, chromosomal abnormalities can be identified. For several decades chromosome analysis has been the golden standard to detect these genomic imbalances. A major drawback of this method is the requirement of short term cultures of fetal cells. In this study we evaluated the combined use of array CGH and flow cytometry FCM, for detection of chromosomal abnormalities, as an alternative for karyotyping.

MethodsIn total 100 spontaneous abortions and mors in utero samples were investigated by karyotyping and array CGH in combination with FCM in order to compare the results for both methods.

ResultsChromosome analysis revealed 17 abnormal karyotypes whereas array CGH in combination with FCM identified 26 aberrations due to the increased test success rate. Karyotyping was unsuccessful in 28% of cases as compared to only two out of hundred samples with inconclusive results for combined array CGH and FCM analysis.

ConclusionThis study convincingly shows that array CGH analysis for detection of numerical and segmental imbalances in combination with flow cytometry for detection of ploidy status has a significant higher detection rate for chromosomal abnormalities as compared to karyotyping of miscarriages samples.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2350-10-89 contains supplementary material, which is available to authorized users.

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Autor: Björn Menten - Katrien Swerts - Barbara Delle Chiaie - Sandra Janssens - Karen Buysse - Jan Philippé - Frank Speleman

Fuente: https://link.springer.com/







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