In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in MiceReportar como inadecuado




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Oxidative Medicine and Cellular Longevity - Volume 2017 2017, Article ID 2689051, 9 pages - https:-doi.org-10.1155-2017-2689051

Research Article

College of Biological Science and Engineering, Fuzhou University, No. 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, China

Laboratory of Radiation Oncology and Radiobiology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fujian Key Laboratory of Tumor Translational Cancer Medicine, The National Clinical Key Specialty Construction Program of China, No. 420 Fuma Road, Fuzhou 350014, China

Food Nutrition Sciences Centre, Zhejiang Gongshang University, Room 407, No. 1 Laboratory Building, No. 149 Jiaogong Road, Xihu District, Hangzhou 310012, China

Correspondence should be addressed to Jianru Pan and Pingfan Rao

Received 31 March 2017; Revised 27 May 2017; Accepted 5 June 2017; Published 19 July 2017

Academic Editor: Ryuichi Morishita

Copyright © 2017 Jianru Pan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

GST-TAT-SOD was the fusion of superoxide dismutase SOD, cell-permeable peptide TAT, and glutathione-S-transferase GST. It was proved to be a potential selective radioprotector in vitro in our previous work. This study evaluated the in vivo radioprotective activity of GST-TAT-SOD against whole-body irradiation. We demonstrated that intraperitoneal injection of 0.5 ml GST-TAT-SOD 2 kU-ml 2 h before the 6 Gy whole-body irradiation in mice almost completely prevented the splenic damage. It could significantly enhance the splenic antioxidant activity which kept the number of splenic white pulp and consequently resisted the shrinkage of the spleen. Moreover, the thymus index, hepatic antioxidant activity, and white blood cell WBC count of peripheral blood in irradiated mice pretreated with GST-TAT-SOD also remarkably increased. Although the treated and untreated irradiated mice showed no significant difference in the growth rate of animal body weight at 7 days postirradiation, the highest growth rate of body weight was observed in the GST-TAT-SOD-pretreated group. Furthermore, GST-TAT-SOD pretreatment increased resistance against 8 Gy whole-body irradiation and enhanced 30 d survival. The overall effect of GST-TAT-SOD seemed to be a bit more powerful than that of amifostine. In conclusion, GST-TAT-SOD would be a safe and potentially promising radioprotector.





Autor: Jianru Pan, Huocong He, Ying Su, Guangjin Zheng, Junxin Wu, Shutao Liu, and Pingfan Rao

Fuente: https://www.hindawi.com/



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