Development of a novel DNA sequencing method not only for hepatitis B virus genotyping but also for drug resistant mutation detectionReportar como inadecuado




Development of a novel DNA sequencing method not only for hepatitis B virus genotyping but also for drug resistant mutation detection - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Medical Genomics

, 6:S15

First Online: 23 January 2013

Abstract

BackgroundIn HBV-infected patients, different genotypes of the hepatitis B virus influence liver disease progression and response to antiviral therapy. Moreover, long-term antiviral therapy will eventually select for drug-resistant mutants. Detection of mutations associated to antiviral therapy and HBV genotyping are essential for monitoring treatment of chronic hepatitis B patients.

ResultsIn this study, a simple method of partial-S gene sequencing using a common PCR amplification was established for genotyping clinical HBV isolates sensitively, which could detect the drug-resistant mutations successfully at the same time.

ConclusionsThe partial S gene sequencing assay developed in this study has potential for application in HBV genotyping and drug resistant mutation detection. It is simpler and more convenient than traditional S gene sequencing, but has nearly the same sensitivity and specificity when compared to S gene sequencing.

Fanjun Wang, Lili Lu contributed equally to this work.

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Autor: Fanjun Wang - Lili Lu - Changshun Yu - Zhanwu Lv - Xuelian Luo - Chao Wan - Zhaohui Hu - Qinyi Zhu - Youping Deng - Chuyu

Fuente: https://link.springer.com/







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