The silence of MUC2 mRNA induced by promoter hypermethylation associated with HBV in Hepatocellular CarcinomaReportar como inadecuado




The silence of MUC2 mRNA induced by promoter hypermethylation associated with HBV in Hepatocellular Carcinoma - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Medical Genetics

, 14:14

First Online: 25 January 2013Received: 27 February 2012Accepted: 23 January 2013

Abstract

BackgroundTo evaluate the promoter methylation status of MUC2 gene and mRNA expression in patients with hepatocellular carcinoma.

MethodsWe analyzed MUC2 methylation by MSP, and MUC2 mRNA by real-time PCR in 74 HCC.

ResultsMUC2 mRNA were lower in HCC tissues Mean -ΔCt = −4.70 than that in Non-HCC tissues Mean -ΔCt = −2.98. Expression of MUC2 was elevated in only 23 31.08% of the 74 HCC patients. MUC2 promoter was hypermethylated in 62.2% 46-74 of HCCs, and in only 18.9% 14-74 of non-tumor samples. MUC2 mRNA were lower in HCC patients with hypermethylation Mean -ΔΔCt = −2.25 than those with demethylation Mean -ΔΔCt = −0.22, and there is a decreased tendency for MUC2 mRNA in HCC patients with promoter hypermethylation p = 0.011. There was a significantly correlation found between MUC2 mRNA and HBV and AFP in HCC. The loss of MUC2 mRNA and hypermethylation could be poor prognostic factors. After treated by 5-Aza-CdR and TSA, we found that MUC2 mRNA induced significantly in 7721, Huh7 and HepG2 cells.

ConclusionThe results suggested that MUC2 mRNA silenced by promoter hypermethylation is associated with high levels HBV in HCC.

KeywordsMUC2 Hepatocellular carcinoma Methylation Electronic supplementary materialThe online version of this article doi:10.1186-1471-2350-14-14 contains supplementary material, which is available to authorized users.

Yang Ling, Jing Zhu, Lu Gao contributed equally to this work.

Download fulltext PDF



Autor: Yang Ling - Jing Zhu - Lu Gao - Yongping Liu - Changtai Zhu - Rong Li - Lixin Wei - Changsong Zhang

Fuente: https://link.springer.com/







Documentos relacionados