Expression of a rice chitinase gene in transgenic banana ‘Gros Michel’, AAA genome group confers resistance to black leaf streak diseaseReport as inadecuate

Expression of a rice chitinase gene in transgenic banana ‘Gros Michel’, AAA genome group confers resistance to black leaf streak disease - Download this document for free, or read online. Document in PDF available to download.

Transgenic Research

, Volume 22, Issue 1, pp 117–130

First Online: 13 July 2012Received: 01 March 2012Accepted: 30 June 2012


Transgenic banana Musa acuminata ‘Gros Michel’ integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %. The transgene insert number per line ranged from 1 to 5 and single transgene insert lines 25 % of all were identified. Considerable delay in disease development up to 63 days post-incoculation over a monitoring period of 108 days occurred in nine lines with extracellularly targeted chitinase out of 17 transgenic lines tested and their necrotic leaf area decreased by 73–94 % compared to the untransformed susceptible control line. Finally, correlation between symptom development and rice chitinase expression was confirmed in two lines by Western analysis. The potential of rice chitinase genes to enhance resistance against M. fijiensis in banana was demonstrated as well as the usefulness of the leaf disk bioassay for early disease screening in transgenic banana lines.

KeywordsBlack leaf streak disease Leaf disk assay Musa spp. Mycosphaerella fijiensis Transgenic banana Gabriella Kovács, László Sági contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1007-s11248-012-9631-1 contains supplementary material, which is available to authorized users.

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Author: Gabriella Kovács - László Sági - Géraldine Jacon - Geofrey Arinaitwe - Jean-Pierre Busogoro - Els Thiry - Hannelore St


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