Evolution of a transposon in Daphnia hybrid genomesReport as inadecuate

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Mobile DNA

, 4:7

First Online: 06 February 2013Received: 20 June 2012Accepted: 06 November 2012


BackgroundTransposable elements play a major role in genome evolution. Their capacity to move and-or multiply in the genome of their host may have profound impacts on phenotypes, and may have dramatic consequences on genome structure. Hybrid and polyploid clones have arisen multiple times in the Daphnia pulex complex and are thought to reproduce by obligate parthenogenesis. Our study examines the evolution of a DNA transposable element named Pokey in the D. pulex complex.

ResultsPortions of Pokey elements inserted in the 28S rRNA genes from various Daphnia hybrids diploids and polyploids were sequenced and compared to sequences from a previous study to understand the evolutionary history of the elements. Pokey sequences show a complex phylogenetic pattern. We found evidence of recombination events in numerous Pokey alleles from diploid and polyploid hybrids and also from non-hybrid diploids. The recombination rate in Pokey elements is comparable to recombination rates previously estimated for 28S rRNA genes in the congener, Daphnia obtusa. Some recombinant Pokey alleles were encountered in Daphnia isolates from multiple locations and habitats.

ConclusionsPhylogenetic and recombination analyses showed that recombination is a major force that shapes Pokey evolution. Based on Pokey phylogenies, reticulation has played and still plays an important role in shaping the diversity of the D. pulex complex. Horizontal transfer of Pokey seems to be rare and hybrids often possess Pokey elements derived from recombination among alleles encountered in the putative parental species. The insertion of Pokey in hotspots of recombination may have important impacts on the diversity and fitness of this transposable element.

KeywordsDaphnia pulex Transposable element Pokey Hybrids Recombination AbbreviationsAaamino acid

Bpbase pair

BSAbovine serum albumin

GARDGenetic Algorithm for Recombination Detection

IGSintergenic spacer

mtDNAmitochondrial DNA

PCRpolymerase chain reaction

RFLPrestriction fragment length polymorphism

SINEsshort interspersed elements

TEstransposable elements

TIRsterminal inverted repeats

Electronic supplementary materialThe online version of this article doi:10.1186-1759-8753-4-7 contains supplementary material, which is available to authorized users.

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Author: Roland Vergilino - Tyler A Elliott - Philippe Desjardins-Proulx - Teresa J Crease - France Dufresne

Source: https://link.springer.com/

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