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Cancer Cell International

, 10:17

First Online: 24 May 2010Received: 13 March 2010Accepted: 24 May 2010


BackgroundThe present study mainly aimed to investigate the direct effects of Endostar ES on the proliferation and radiosensitivity of human lung squamous cancer cell line H-520.

ResultsES significantly inhibited H-520 cell proliferation in a time- and dose-dependent manner. According to the colony-forming assays, ES could increase the H-520 cell radiosensitivity. ES induced cell apoptosis, the apoptosis rate increased with the raise of ES concentration. Irradiation induced significantly higher apoptosis rate in ES-treated H-520 cells than non-treated H-520 cells. ES induced cell cycle distribution and G0-G1 arrest in H-520 cells, whereas irradiation induced G2-M arrest. The phospho-p38-MAPK and p-Akt protein levels were decreased in H-520 cells after ES treatment. Furthermore, activated caspase protein level increased and Bcl-2 protein levels decreased after treatment with ES and irradiation.

ConclusionES significantly enhanced the sensitivity of H-520 cells to irradiation by inhibition of cellular proliferation, promotion of cell apoptosis and redistribution of cell cycle, possibly via deactivation of Akt pathway. The present study supports the possibility to use the combination of ES and ionizing irradiation to treat patients with lung squamous cell cancer in clinics.

AbbreviationsED recombinant human endostatin

ES Endostar

PE plating efficiency

SF survival fraction

SER sensitization enhancement ratio

D 0 mean lethal dose

mean inactivation doseD q : quasithreshold dose

N extrapolation number

SF 2 surviving fraction after 2Gy irradiation

MTT methyl thiazolyl tetrazolium

FCM flow cytometry

NSCLC non-small cell lung cancer

HUVEC S human umbilical vein endothelial cells.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-10-17 contains supplementary material, which is available to authorized users.

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Autor: Zhen Y You - Yong Zhao - Feng Liu - Ying D Zhang - Jun J Wang


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