Methylation analysis of the phosphates and tensin homologue on chromosome 10 gene PTEN in multiple myelomaReportar como inadecuado




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Clinical Epigenetics

, 6:16

First Online: 20 August 2014Received: 24 April 2014Accepted: 12 August 2014

Abstract

BackgroundAberrant DNA methylation of promoter region CpG islands is an alternative mechanism that leads to genetic defects in the inactivation of tumor suppressor genes during myelomagenesis. The aim of this study was to examine the promoter methylation status of the phosphates and tensin homologue on chromosome 10 PTEN gene in a cohort of multiple myeloma patients.

FindingsThe PTEN gene was hypermethylated in 7 out of 58 12% primary myeloma samples. The correlation between functional inactivation and PTEN mRNA levels was not statistically significant. The multiple myeloma subgroup with an aberrant PTEN status had a prevalence of the component IgG, Salmon Durie stage I, lower lactate dehydrogenase levels, intermediate-standard cytogenetic risk and longer overall survival with the respect to the unmethylated subgroup.

ConclusionsThis is the first report demonstrating the presence of PTEN promoter hypermethylation in multiple myeloma.

KeywordsPromoter hypermethylation multiple myeloma Akt pathway PTEN AbbreviationsAMLacute myeloid leukemia

B-ALLB lymphoblastic leukemia

BMbone marrow

CMLchronic myeloid leukemia

FISHfluorescent in situ hybridization

LDHlactate dehydrogenase

MPDmyeloproliferative disorders

MGUSmonoclonal gammopathy of undetermined significance

MMmultiple myeloma

MSPmethylation specific polymerase chain reaction

OSoverall survival.

Electronic supplementary materialThe online version of this article doi:10.1186-1868-7083-6-16 contains supplementary material, which is available to authorized users.

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Autor: Giovanna Piras - Maria Monne - Angelo D Palmas - Anna Calvisi - Rosanna Asproni - Francesco Vacca - Laura Pilo - Attilio 

Fuente: https://link.springer.com/







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