Effects of myenteric denervation on extracellular matrix fibers and mast cell distribution in normal stomach and gastric lesionsReportar como inadecuado

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Cancer Cell International

, 10:18

First Online: 22 June 2010Received: 10 April 2010Accepted: 22 June 2010


BackgroundIn this study the effect of myenteric denervation induced by benzalconium chloride BAC on distribution of fibrillar components of extracellular matrix ECM and inflammatory cells was investigated in gastric carcinogenesis induced by N-methyl-N-nitro-N-nitrosoguanidine MNNG. Rats were divided in four experimental groups: non-denervated I and denervated stomach II without MNNG treatment; non-denervated III and denervated stomachs IV treated with MNNG. For histopathological, histochemical and stereological analysis, sections of gastric fragments were stained with Hematoxylin-Eosin, Picrosirius-Hematoxylin, Gomori reticulin, Weigert-s Resorcin-Fuchsin, Toluidine Blue and Alcian-Blue-Safranin AB-SAF.

ResultsBAC denervation causes an increase in the frequency of reticular and elastic fibers in the denervated group II compared to the non-denervated stomachs group I. The treatment of the animals with MNNG induced the development of adenocarcinomas in non-denervated and denervated stomachs groups III and IV, respectively with a notable increase in the relative volume of the stroma, the frequency of reticular fibers and the inflammatory infiltrate that was more intense in group IV. An increase in the frequency of elastic fibers was observed in adenocarcinomas of denervated group IV compared to the non-denervated stomachs group III that showed degradation of these fibers. The development of lesions groups III and IV was also associated with an increase in the mast cell population, especially AB and AB-SAF positives, the latter mainly in the denervated group IV.

ConclusionsThe results show a strong association in the morphological alteration of the ECM fibrillar components, the increased density of mast cells and the development of tumors induced by MNNG in the non-denervated rat stomach or denervated by BAC. This suggests that the study of extracellular and intracellular components of tumor microenvironment contributes to understanding of tumor biology by action of myenteric denervation.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-10-18 contains supplementary material, which is available to authorized users.

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Autor: Cássia F Estofolete - Carla Botelho-Machado - Sebastião R Taboga - Sérgio Zucoloto - Ana Cláudia Polli-Lopes - Cristia

Fuente: https://link.springer.com/

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