The most common Chinese rhesus macaque MHC class I molecule shares peptide binding repertoire with the HLA-B7 supertypeReportar como inadecuado




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Immunogenetics

, Volume 62, Issue 7, pp 451–464

First Online: 18 May 2010Received: 20 March 2010Accepted: 19 April 2010

Abstract

Of the two rhesus macaque subspecies used for AIDS studies, the Simian immunodeficiency virus-infected Indian rhesus macaque Macaca mulatta is the most established model of HIV infection, providing both insight into pathogenesis and a system for testing novel vaccines. Despite the Chinese rhesus macaque potentially being a more relevant model for AIDS outcomes than the Indian rhesus macaque, the Chinese-origin rhesus macaques have not been well-characterized for their major histocompatibility complex MHC composition and function, reducing their greater utilization. In this study, we characterized a total of 50 unique Chinese rhesus macaques from several varying origins for their entire MHC class I allele composition and identified a total of 58 unique complete MHC class I sequences. Only nine of the sequences had been associated with Indian rhesus macaques, and 28-58 48.3% of the sequences identified were novel. From all MHC alleles detected, we prioritized Mamu-A1*02201 for functional characterization based on its higher frequency of expression. Upon the development of MHC-peptide binding assays and definition of its associated motif, we revealed that this allele shares peptide binding characteristics with the HLA-B7 supertype, the most frequent supertype in human populations. These studies provide the first functional characterization of an MHC class I molecule in the context of Chinese rhesus macaques and the first instance of HLA-B7 analogy for rhesus macaques.

KeywordsRhesus macaque MHC HLA CTL Electronic supplementary materialThe online version of this article doi:10.1007-s00251-010-0450-3 contains supplementary material, which is available to authorized users.

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Autor: Christopher Solomon - Scott Southwood - Ilka Hoof - Richard Rudersdorf - Bjoern Peters - John Sidney - Clemencia Pinilla -

Fuente: https://link.springer.com/







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