The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cellsReportar como inadecuado




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Cancer Cell International

, 10:24

First Online: 28 July 2010Received: 06 January 2010Accepted: 28 July 2010

Abstract

We have shown that the microtopography mT underlying colon cancer changes as a tumor de-differentiates. We distinguish the well-differentiated mT based on the increasing number of -pits- and poorly differentiated mT on the basis of increasing number of -posts.- We investigated Rho A as a mechanosensing protein using mT features derived from those observed in the ECM of colon cancer. We evaluated Rho A activity in less-tumorogenic Caco-2 E and more tumorigenic SW620 colon cancer cell-lines on microfabricated pits and posts at 2.5 μm diameter and 200 nm depth-height. In Caco-2 E cells, we observed a decrease in Rho A activity as well as in the ratio of G-F actin on surfaces with either pits or posts but despite this low activity, knockdown of Rho A led to a significant decrease in confined motility suggesting that while Rho A activity is reduced on these surfaces it still plays an important role in controlling cellular response to barriers. In SW620 cells, we observed that Rho A activity was greatest in cells plated on a post microtopography which led to increased cell motility, and an increase in actin cytoskeletal turnover.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-10-24 contains supplementary material, which is available to authorized users.

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Autor: Rebecca Rapier - Jameela Huq - Ramana Vishnubhotla - Marinka Bulic - Cecile M Perrault - Vitali Metlushko - Michael Cho -

Fuente: https://link.springer.com/







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