Chronic allergen challenge induces bronchial mast cell accumulation in BALB-c but not C57BL-6 mice and is independent of IL-9Reportar como inadecuado




Chronic allergen challenge induces bronchial mast cell accumulation in BALB-c but not C57BL-6 mice and is independent of IL-9 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Immunogenetics

, Volume 62, Issue 8, pp 499–506

First Online: 18 May 2010Received: 25 January 2010Accepted: 27 April 2010

Abstract

As genetically engineered mutant mice deficient in single genes are usually generated on a C57BL-6 background, to study mast cell trafficking in mutant mice, we initially investigated whether mast cells accumulated in bronchi in C57BL-6 mice challenged with OVA allergen acutely or chronically for 1 to 3 months. The total number of bronchial mast cells were quantitated using toluidine blue staining in airways of different sizes, i.e. , small <90 µm, medium 90–155 µm, or large >150 µm airways. Non-OVA challenged and acute OVA challenged mice C57BL-6 and BALB-c had no detectable bronchial mast cells. Chronic OVA challenge in BALB-c mice for 1 or 3 months induced a significant increase in the number of bronchial mast cells in small-, medium-, and large-sized airways but minimal change in the number of bronchial mast cells in C57BL-6 mice. Both BALB-c and C57BL-6 mice developed significant lung eosinophilia following acute or chronic OVA challenge. Studies of IL-9-deficient mice on a BALB-c background demonstrated a significant increase in the number of bronchial mast cells in IL-9-deficient mice suggesting that IL-9 was not required for the bronchial accumulation of mast cells. Overall, these studies demonstrate that the chronic OVA challenge protocol we have utilized in BALB-c mice provides a model to study the mechanism of bronchial mast cell accumulation and that bronchial mast cell accumulation in chronic OVA challenged mice is independent of IL-9 in this model.

KeywordsBALB-c C57BL-6 Mast cell Eosinophil  Download fulltext PDF



Autor: Suzan Pae - Jae Youn Cho - Shanna Dayan - Marina Miller - Alan D. Pemberton - David H. Broide

Fuente: https://link.springer.com/







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