DNA methylation differences between in vitro- and in vivo-conceived children are associated with ART procedures rather than infertilityReportar como inadecuado




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Clinical Epigenetics

, 7:41

First Online: 08 April 2015Received: 02 December 2014Accepted: 10 March 2015

Abstract

BackgroundWe, and others, have demonstrated previously that there are differences in DNA methylation and transcript levels of a number of genes in cord blood and placenta between children conceived using assisted reproductive technologies ART and children conceived in vivo. The source of these differences the effect of ART versus the underlying infertility has never been determined in humans. In this study, we have attempted to resolve this issue by comparing placental DNA methylation levels at 37 CpG sites in 16 previously identified candidate genes in independent populations of children conceived in vivo ‘fertile control’ group with ART children conceived from two groups: either autologous oocytes with infertility in one or both parents ‘infertile ART’ group or donor oocytes obtained from young fertile donors without male infertility ‘donor oocyte ART’ group.

ResultsOf the 37 CpG sites analyzed, significant differences between the three groups were found in 11 CpGs 29.73 %, using ANOVA. Tukey’s post hoc test on the significant results indicated that seven 63.63 % of these differences were significant between the donor oocyte ART and fertile control groups. In addition, 20 of the 37 CpGs analyzed had been identified as differentially methylated between ART and fertile control groups in an independent population in a prior study. Of these 20 CpG sites, 9 also showed significant differences in the present population. An additional 9 CpGs were found to be significantly different between the two groups. Of these 18 candidate CpGs, 12 CpGs in seven candidate genes also showed significant differences in placental DNA methylation levels between the donor oocyte ART and fertile control groups.

ConclusionsThese data suggest strongly that the DNA methylation differences observed between ART and in vivo conceptions are associated with some aspect of ART protocols, not simply the underlying infertility.

KeywordsDNA methylation Assisted reproduction Donor oocytes Infertility Placenta Electronic supplementary materialThe online version of this article doi:10.1186-s13148-015-0071-7 contains supplementary material, which is available to authorized users.

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Autor: Sisi Song - Jayashri Ghosh - Monica Mainigi - Nahid Turan - Rachel Weinerman - May Truongcao - Christos Coutifaris - Carmen

Fuente: https://link.springer.com/







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