Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cellsReportar como inadecuado




Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cancer Cell International

, 13:26

First Online: 13 March 2013Received: 23 July 2012Accepted: 07 March 2013

Abstract

BackgroundBeclin1 is a well-known key regulator of autophagy, which is also a haploinsufficient tumor suppressor. Current studies revealed that down-regulation or monoallelic deletions of Beclin1 were frequently found in various cancers. The purpose of this study was to investigate the effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of pancreatic cancer cells.

MethodsBeclin1 expression was inhibited by siRNA transduction and gene expression was determined by Real-time PCR and Western blot. The effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of Miapaca2 cells were analyed through LC3 expression, cell viability, cell cycle and apoptosis by using Western blot.

ResultsWe observed that Beclin1 silence promoted microtubule-associated protein 1 light chain 3-II LC3-II protein formation and increased punctate fluorescent signals in Miapaca2 cells transfected with green fluorescent protein GFP-tagged LC3. Beclin1 inhibition showed a greater suppressive effect on Gemcitabine-induced apoptosis of Miapaca2 cells.

ConclusionOur data suggested that Beclin1 silence not only up-adjusted autophagy process, but also played an important role in the regulation of apoptosis. Beclin1 inhibition could inhibit apoptosis signaling induced by Gemcitabine in Miapaca2 cells.

AbbreviationsLC3microtubule-associated protein 1 Light Chain 3.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2867-13-26 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Xiaoshu Li - Jun Yan - Lisheng Wang - Fengjun Xiao - Yuefeng Yang - Xiaozhong Guo - Hua Wang

Fuente: https://link.springer.com/







Documentos relacionados