Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin GlycoproteinReportar como inadecuado

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International Journal of Cell Biology - Volume 2014 2014, Article ID 725928, 13 pages -

Research Article

IRCCS-G.B. Bietti Foundation, Rome, Italy

Institute of Histology and Embryology, School of Medicine “A. Gemelli”, Catholic University of the Sacred Heart, Rome, Italy

Laboratory of Developmental Neuroscience and Neural Plasticity, University Campus Bio-Medico, Rome, Italy

Laboratory of Ophthalmology, IRCCS-G.B. Bietti Foundation, Via Alvaro del Portillo 21, 00128 Rome, Italy

Received 11 October 2013; Accepted 4 November 2013; Published 30 January 2014

Academic Editor: Alessio D’Alessio

Copyright © 2014 Bijorn Omar Balzamino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Both Reelin and Nerve Growth Factor NGF exert crucial roles in retinal development. Retinogenesis is severely impaired in E-reeler mice, a model of Reelin deficiency showing specific Green Fluorescent Protein expression in Rod Bipolar Cells RBCs. Since no data are available on Reelin and NGF cross-talk, NGF and - expression was investigated in retinas from E-reeler versus control mice, by confocal microscopy, Western blotting, and real time PCR analysis. A scattered increase of NGF protein was observed in the Ganglion Cell Layer and more pronounced in the Inner Nuclear Layer INL. A selective increase of was detected in most of RBCs and in other cell subtypes of INL. On the contrary, a slight trend towards a decrease was detected for , albeit not significant. Confocal data were validated by Western blot and real time PCR. Finally, the decreased - ratio, representative of increase, significantly correlated with E-reeler versus E-control. These data indicate that NGF- is affected in E-reeler retina and that might represent the main NGF receptor involved in the process. This first NGF- characterization suggests that E-reeler might be suitable for exploring Reelin-NGF cross-talk, representing an additional information source in those pathologies characterized by retinal degeneration.

Autor: Bijorn Omar Balzamino, Filippo Biamonte, Graziana Esposito, Ramona Marino, Francesca Fanelli, Flavio Keller, and Alessandra



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