Inflammation triggers specific microRNA profiles in human adipocytes and macrophages and in their supernatantsReportar como inadecuado

Inflammation triggers specific microRNA profiles in human adipocytes and macrophages and in their supernatants - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Clinical Epigenetics

, 7:49

First Online: 24 April 2015Received: 27 January 2015Accepted: 07 April 2015


BackgroundThe relevance of microRNAs miRNAs in adipose tissue is increasingly recognized, being intrinsically linked to different pathways, including obesity-related inflammation. In this study, we aimed to characterize the changes induced by inflammation on the miRNA pattern of human adipocytes and macrophages. Therefore, an extensive profile of 754 common miRNAs was assessed in cells human primary mature adipocytes, and the macrophage-like cell line THP-1 and in their supernatants SN using TaqMan low-density arrays. These profiles were evaluated at the baseline and after administration of lipopolysaccharide LPS, 10 ng-ml and LPS-conditioned medium from M1 macrophages MCM, 5%. The miRNAs that experienced the most dramatic changes were studied in subcutaneous human adipose tissue before and approximately 2 years after bariatric surgery-induced weight loss.

ResultsDifferentiated adipocytes expressed 169 miRNAs, being 85 detectable in the SN. In M1 macrophages, 183 miRNAs were detected, being 106 also present in the SN. Inflammation led to an increased number of miRNAs detectable in cells and in their SNs in both adipocytes +8.3% and +24.7% and M1 macrophages +1.4% and +5%, respectively. Indeed, under inflammatory conditions, adipocytes and M1 macrophages shared the expression of 147 +9% miRNAs, and 100 +41% common miRNAs were found in their SNs. Twelve of these factors were also linked to inflammation in whole adipose tissue from obese subjects. Interestingly, miR-221 2-fold, P = 0.002, miR-222 2.5-fold, P = 0.04, and miR-155 5-fold, P = 0.015 were increased in inflamed adipocytes and in their SNs 15-, 6-, and 4-fold, respectively, all P < 0.001. Furthermore, their expressions in human adipose tissue concordantly decreased after weight loss −51%, P = 0.003, −49%, P = 0.03, and −54.4%, P = 0.005, respectively.

ConclusionsInflammation induces a specific miRNA pattern in adipocytes and M1 macrophages, with impact on the physiopathology of obesity-induced inflammation of adipose tissue. The crosstalk between cells should be investigated further.

KeywordsmicroRNAs RT-PCR Profiling Macrophages Adipocytes Adipose tissue Obesity Inflammation Electronic supplementary materialThe online version of this article doi:10.1186-s13148-015-0083-3 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Francisco José Ortega - María Moreno - Josep María Mercader - José María Moreno-Navarrete - Núria Fuentes-Batllevell


Documentos relacionados