A pilot proof-of-principle study to compare fresh and vitrified cycle preimplantation genetic screening by chromosome microarray and next generation sequencingReportar como inadecuado




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Molecular Cytogenetics

, 9:25

Clinical genetics

Abstract

BackgroundSingle embryo transfer SET has been utilized as a strategy to reduce the chance of multifetal gestations in in vitro fertilization IVF but lower pregnancy rate remains a concern. Recent studies showed that favorable outcome regarding SET can be achieved by selecting embryos with -more normal- genetic components. We explored the use of rapid array comparative genomic hybridization aCGH to select blastocysts for fresh SET and compared with the protocols adopting vitrified ultrarapidly frozen embryo transfer cycle. Validation of the rapid protocol of aCGH and comparison of the result with the regular protocol of aCGH and next generation sequencing NGS are also performed.

ResultsFirst-time IVF patients with normal karyotype n = 21 were enrolled for elective fresh SET cycle n = 8; designated as fresh SET group or vitrified embryo transfer cycle n = 13; designated as vitrified ET group coupling with comprehensive chromosomal screening by a 9-h rapid aCGH from Day 5 trophectoderm TE biopsy. In fresh SET group, 86 blastocysts 10.8 blastocysts-patient were biopsied and analyzed. Aneuploidy was detected in 53.5 % 46-86 of the biopsied blastocysts. All patients had a single embryo transferred on the following day. The clinical pregnancy rate was 87.5 % 7-8 and the ongoing pregnancy rate was 62.5 % 5-8. In vitrified ET group, 58 blastocysts 4.5 blastocysts-patient were biopsied and 56 blastocysts were analyzed. Aneuploidy was detected in 39.3 % 22-56 of biopsies. The patients accepted for SET or double embryos transfer DET in non-stimulated cycles. The clinical pregnancy rate and the ongoing pregnancy rate was 76.9 % 10-13 and 53.8 % 7-13 respectively. Spontaneous abortions occurred in both of the two patient groups. In the series of fresh SET group, no twin pregnancy was noted and at least one healthy baby had been born at gestational age GA 37 weeks when submission. The results of PGS by rapid aCGH, regular aCGH and NGS were comparable in most occasions.

ConclusionThis study evaluates the use of rapid aCGH to select blastocysts for fresh SET and demonstrates its feasibility in a real clinical IVF program. A successful livebirth is achieved and the favorable outcome is superior to the protocol adopting vitrified ET cycle in our own setting. Additional studies are needed to verify this pilot data and validate its application in large randomized trials.

KeywordsChromosomal microarray Aneuploidy Fresh embryo transfer SET PGS AbbreviationsaCGHarray comparative genomic hybridization

ARTartificial reproductive technology

DETdouble embryo transfer

ETembryo transfer

FISHfluorescence in situ hybridization

GAgestation age

ICMinner cell mass

ICSIintracytoplasmic sperm injection

ISPsion Sphere Particles

IVFin vitro fertilization

NGSnext generation sequencing

PGSpreimplantation genetic screening

qPCRquantitative polymerase chain reaction

SETsingle embryo transfer

TEtrophectoderm

WGAwhole genome amplification

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Autor: Gwo-Chin Ma - Hsin-Fu Chen - Yu-Shih Yang - Wen-Hsiang Lin - Feng-Po Tsai - Chi-Fang Lin - Chi Chiu - Ming Chen

Fuente: https://link.springer.com/







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