Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer modelReportar como inadecuado




Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Clinical Epigenetics

, 8:34

Cancer epigenetics and diagnostics

Abstract

BackgroundEpigenetic regulation has emerged to be the critical steps for tumorigenesis and metastasis. Multiple histone methyltransferase and demethylase have been implicated as tumor suppressors or oncogenes recently. But the key epigenomic events in cancer cell transformation still remain poorly understood.

MethodsA breast cancer transformation model was established via stably expressing three oncogenes in primary breast epithelial cells. Chromatin immunoprecipitation followed by the next-generation sequencing of histone methylations was performed to determine epigenetic events during transformation. Western blot, quantitative RT-PCR, and immunostaining were used to determine gene expression in cells and tissues.

ResultsHistones H3K9me2 and me3, two repressive marks of transcription, decrease in in vitro breast cancer cell model and in vivo clinical tissues. A survey of enzymes related with H3K9 methylation indicated that KDM3A-JMJD1A, a demethylase for H3K9me1 and me2, gradually increases during cancer transformation and is elevated in patient tissues. KDM3A-JMJD1A deficiency impairs the growth of tumors in nude mice and transformed cell lines. Genome-wide ChIP-seq analysis reveals that the boundaries of decreased H3K9me2 large organized chromatin K9 modifications LOCKs are enriched with cancer-related genes, such as MYC and PAX3. Further studies show that KDM3A-JMJD1A directly binds to these oncogenes and regulates their transcription by removing H3K9me2 mark.

ConclusionsOur study demonstrates reduction of histones H3K9 me2 and me3, and elevation of KDM3A-JMJD1A as important events for breast cancer, and illustrates the dynamic epigenomic mechanisms during breast cancer transformation.

KeywordsH3K9 methylation Epigenomics Breast cancer transformation KDM3A Transcription regulation Electronic supplementary materialThe online version of this article doi:10.1186-s13148-016-0201-x contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Quan-Yi Zhao - Pin-Ji Lei - Xiaoran Zhang - Jun-Yi Zheng - Hui-Yi Wang - Jiao Zhao - Yi-Ming Li - Mei Ye - Lianyun Li - G

Fuente: https://link.springer.com/







Documentos relacionados