Cell therapy for full-thickness wounds: are fetal dermal cells a potential sourceReportar como inadecuado




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Cell and Tissue Research

, Volume 364, Issue 1, pp 83–94

First Online: 09 October 2015Received: 04 February 2015Accepted: 03 September 2015

Abstract

The application of autologous dermal fibroblasts has been shown to improve burn wound healing. However, a major hurdle is the availability of sufficient healthy skin as a cell source. We investigated fetal dermal cells as an alternative source for cell-based therapy for skin regeneration. Human hFF, porcine fetal pFF or autologous dermal fibroblasts AF were seeded in a collagen–elastin substitute Novomaix, NVM, which was applied in combination with an autologous split thickness skin graft STSG to evaluate the effects of these cells on wound healing in a porcine excisional wound model. Transplantation of wounds with NVM+hFF showed an increased influx of inflammatory cells e.g., neutrophils, macrophages, CD4 and CD8 lymphocytes compared to STSG, acellular NVM Acell-NVM and NVM+AF at post-surgery days 7 and-or 14. Wounds treated with NVM+pFF presented only an increase in CD8 lymphocyte influx. Furthermore, reduced alpha-smooth muscle actin αSMA expression in wound areas and reduced contraction of the wounds was observed with NVM+AF compared to Acell-NVM. Xenogeneic transplantation of NVM+hFF increased αSMA expression in wounds compared to NVM+AF. An improved scar quality was observed for wounds treated with NVM+AF compared to Acell-NVM, NVM+hFF and NVM+pFF at day 56. In conclusion, application of autologous fibroblasts improved the overall outcome of wound healing in comparison to fetal dermal cells and Acell-NVM, whereas application of fetal dermal fibroblasts in NVM did not improve wound healing of full-thickness wounds in a porcine model. Although human fetal dermal cells demonstrated an increased immune response, this did not seem to affect scar quality.

KeywordsFetal fibroblasts Mesenchymal stem cells Allogeneic-xenogeneic transplantation Full-thickness wounds Dermal substitutes  Download fulltext PDF



Autor: J. J. Akershoek - M. Vlig - W. Talhout - B. K. H. L. Boekema - C. D. Richters - R. H. J. Beelen - K. M. Brouwer - E

Fuente: https://link.springer.com/







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