Expression and localization of matrix metalloproteinases MMP-2 -7 -9 and their tissue inhibitors TIMP-2 -3 in the chicken oviduct during maturationReportar como inadecuado




Expression and localization of matrix metalloproteinases MMP-2 -7 -9 and their tissue inhibitors TIMP-2 -3 in the chicken oviduct during maturation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cell and Tissue Research

, Volume 364, Issue 1, pp 185–197

First Online: 22 September 2015Received: 20 July 2015Accepted: 28 August 2015

Abstract

Although participation of matrix metalloproteinases MMPs in reproductive tract remodeling has been strongly suggested in mammalian species, the role of MMPs in the avian oviduct has received little attention. To gain a better understanding of the potential role of the MMP system in avian oviduct development, mRNA and protein expression, localization of selected MMPs and their tissue inhibitors TIMPs, and gelatinolytic activity in the oviduct of growing chickens were examined. The oviducts were collected from Hy-Line Brown hens before 10, 12, 14 and 16 weeks of age and after week 17 the onset of egg laying. The MMP-2 -7 -9 and TIMP-2 and -3 genes were found to be differentially expressed in all examined oviductal sections: the infundibulum, magnum, isthmus and shell gland on both mRNA by real time polymerase chain reaction and protein by western blotting and immunohistochemistry levels. In the course of oviduct development, the relative expression of all genes decreased in most sections. Protein level of MMP-9 was diminished, while MMP-7 and TIMP-3 were elevated in the oviduct of growing birds. MMP-2 and TIMP-2 protein levels remained constant, with a slight increase in MMP-2 concentration just before reaching maturity. The relative activity of MMP-2 and -9 assessed by gelatin zymography was higher P < 0.05, P < 0.01 in immature birds compared with adults. Immunohistochemistry demonstrated cell- and tissue-specific localization of MMPs and TIMPs in the wall of the chicken oviduct. We concluded that changes in the expression of examined MMPs and their inhibitors, as well as alterations in MMP activity occurring simultaneously with changes in the morphology of the chicken oviduct, suggest the involvement of the MMP system in the proper development and functioning of this organ. Mechanisms regulating the expression and activity of MMPs require further clarification.

KeywordsMMPs TIMPs Oviduct Development Chicken  Download fulltext PDF



Autor: Agnieszka Leśniak-Walentyn - Anna Hrabia

Fuente: https://link.springer.com/







Documentos relacionados