Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid FibroblastsReportar como inadecuado




Piper betle L. Modulates Senescence-Associated Genes Expression in Replicative Senescent Human Diploid Fibroblasts - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2017 2017, Article ID 6894026, 9 pages - https:-doi.org-10.1155-2017-6894026

Research Article

Department of Biochemistry, Universiti Kebangsaan Malaysia Medical Centre, Level 17, Preclinical Building, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia

Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Level 18, Preclinical Building, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia

Correspondence should be addressed to Suzana Makpol

Received 22 November 2016; Revised 31 March 2017; Accepted 13 April 2017; Published 17 May 2017

Academic Editor: Adair Santos

Copyright © 2017 Lina Wati Durani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Piper betle PB is a traditional medicine that is widely used to treat different diseases around Asian region. The leaf extracts contain various bioactive compounds, which were reported to have antidiabetic, antibacterial, anti-inflammatory, antioxidant, and anticancer effects. In this study, the effect of PB aqueous extracts on replicative senescent human diploid fibroblasts HDFs was investigated by determining the expressions of senescence-associated genes using quantitative PCR. Our results showed that PB extracts at 0.4 mg-ml can improve cell proliferation of young 143%, presenescent 127.3%, and senescent 157.3% HDFs. Increased expressions of PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were observed in senescent HDFs compared to young and-or presenescent HDFs. Treatment with PB extracts modulates the transcriptional profile changes in senescent HDFs. By contrast, expressions of SOD1 increased, whereas GPX1, PRDX6, TP53, CDKN2A, PAK2, and MAPK14 were decreased in PB-treated senescent HDFs compared to untreated senescent HDFs. In conclusion, this study indicates the modulation of PB extracts on senescence-associated genes expression of replicative senescent HDFs. Further studies warrant determining the mechanism of PB in modulating replicative senescence of HDFs through these signaling pathways.





Autor: Lina Wati Durani, Shy Cian Khor, Jen Kit Tan, Kien Hui Chua, Yasmin Anum Mohd Yusof, and Suzana Makpol

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados