The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophiesReportar como inadecuado




The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Histochemistry and Cell Biology

, Volume 146, Issue 5, pp 627–634

First Online: 09 July 2016Accepted: 23 June 2016

Abstract

Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus EDL, from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin LF-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm muscle tissue correlated highly r ≥ 0.9 with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.

KeywordsLipofuscin Quantification Image analysis Skeletal muscle Mdx mouse Muscular dystrophy  Download fulltext PDF



Autor: Yoshiko Nakae - Peter J. Stoward

Fuente: https://link.springer.com/







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