Protective Effects of Selol Against Sodium Nitroprusside-Induced Cell Death and Oxidative Stress in PC12 CellsReportar como inadecuado




Protective Effects of Selol Against Sodium Nitroprusside-Induced Cell Death and Oxidative Stress in PC12 Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Neurochemical Research

, Volume 41, Issue 12, pp 3215–3226

First Online: 02 September 2016Received: 13 April 2016Revised: 11 August 2016Accepted: 24 August 2016

Abstract

Selol is an organic selenitetriglyceride formulation containing selenium at +4 oxidation level that can be effectively incorporated into catalytic sites of of Se-dependent antioxidants. In the present study, the potential antioxidative and cytoprotective effects of Selol against sodium nitroprusside SNP-evoked oxidative-nitrosative stress were investigated in PC12 cells and the underlying mechanisms analyzed. Spectrophoto- and spectrofluorimetic methods as well as fluorescence microscopy were used in this study; mRNA expression was quantified by real-time PCR. Selol dose-dependently improved the survival and decreased the percentage of apoptosis in PC12 cells exposed to SNP. To determine the mechanism of this protective action, the effect of Selol on free radical generation and on antioxidative potential was evaluated. Selol offered significant protection against the elevation of reactive oxidative species ROS evoked by SNP. Moreover, this compound restored glutathione homeostasis by ameliorating the SNP-evoked disturbance of GSH-GSSG ratio. The protective effect exerted by Selol was associated with the prevention of SNP-mediated down-regulation of antioxidative enzymes: glutathione peroxidase Se-GPx, glutathione reductase GR, and thioredoxin reductase TrxR. Finally, GPx inhibition significantly abolished the cytoprotective effect of Selol. In conclusion, these results suggest that Selol effectively protected PC12 cells against SNP-induced oxidative damage and death by adjusting free radical levels and antioxidant system, and suppressing apoptosis. Selol could be successfully used in the treatments of diseases that involve oxidative stress and resulting apoptosis.

KeywordsAntioxidative defense Cytoprotection Oxidative-nitrosative stress Organic selenium compound Selenoenzymes Selol  Download fulltext PDF



Autor: Agnieszka Dominiak - Anna Wilkaniec - Piotr Wroczyński - Henryk Jęśko - Agata Adamczyk

Fuente: https://link.springer.com/



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