GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase.Reportar como inadecuado




GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 BIOC - Laboratoire de Biochimie de l-Ecole polytechnique

Abstract : GEK1, an Arabidopsis thaliana gene product, was recently identified through its involvement in ethanol tolerance. Later, this protein was shown to display 26% strict identity with archaeal d-Tyr-tRNATyr deacylases. To determine whether it actually possessed deacylase activity, the product of the GEK1 open reading frame was expressed in Escherichia coli from a multi-copy plasmid. Purified GEK1 protein contains two zinc ions and proves to be a broad-specific, markedly active d-aminoacyl-tRNA deacylase in vitro. Moreover, GEK1 expression is capable of functionally compensating in E. coli for the absence of endogeneous d-Tyr- tRNATyr deacylase. Possible connections between exposure of plants to ethanol-acetaldehyde and misaminoacylation of tRNA by d-amino acids are considered.





Autor: Sandra Wydau - Maria-Laura Ferri-Fioni - Sylvain Blanquet - Pierre Plateau -

Fuente: https://hal.archives-ouvertes.fr/



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