Isolation and characterization of antimutagenic components of Glycyrrhiza aspera against N-methyl-N-nitrosoureaReportar como inadecuado




Isolation and characterization of antimutagenic components of Glycyrrhiza aspera against N-methyl-N-nitrosourea - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Genes and Environment

, 39:5

First Online: 06 January 2017Received: 12 May 2016Accepted: 08 December 2016

Abstract

BackgroundA powdered ethanolic extract of Glycyrrhiza aspera root exhibits antimutagenic activity against N-methyl-N-nitrosourea MNU based on the Ames assay with Salmonella typhimurium TA1535. The aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of G. aspera root.

ResultsThe powdered ethanolic extract of G. aspera root was sequentially suspended in n-hexane, carbon tetrachloride, dichloromethane, ethyl acetate, and ethanol, and each solvent soluble fraction and the residue were assayed for antimutagenic activity against MNU in S. typhimurium TA1535. The dichloromethane soluble fraction exhibited the highest antimutagenicity and was fractionated several times by silica gel chromatography. The fraction with the highest antimutagenic activity was further purified using HPLC, and the fractions were assayed for antimutagenicity against MNU in S. typhimurium TA1535. Finally, five components with antimutagenic activity against MNU were identified as glyurallin A, glyasperin B, licoricidin, 1-methoxyphaseollin, and licoisoflavone B.

ConclusionsThe five components were demonstrated to possess an antigenotoxic effect against carcinogenic MNU for the first time. It is important to prevent DNA damage by N-nitrosamines for cancer chemoprevention.

KeywordsGlyurallin A Glyasperin B Licoricidin 1-Methoxyphaseollin Licoisoflavone B AbbreviationMNUN-Methyl-N-nitrosourea

Electronic supplementary materialThe online version of this article doi:10.1186-s41021-016-0068-2 contains supplementary material, which is available to authorized users.

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Autor: Keiko Inami - Yusuke Mine - Jin Tatsuzaki - Chihiro Mori - Masataka Mochizuki

Fuente: https://link.springer.com/







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