Practical Implication of Chromatographic Method for Estimation of Aceclofenac and Pregabalin in Bulk and Pharmaceutical Dosage FormsReportar como inadecuado

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Chromatography Research International - Volume 2014 2014, Article ID 643027, 5 pages -

Research ArticleDepartment of Pharmaceutical Chemistry, Sardar Patel College of Pharmacy, Bakrol, Anand, Gujarat 388120, India

Received 21 July 2014; Accepted 29 October 2014; Published 16 December 2014

Academic Editor: Toyohide Takeuchi

Copyright © 2014 Hitendrakumar D. Gelani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Aceclofenac and Pregabalin in combination significantly reduce pain as compared to individual drug in chronic low back pain. Literature reveals that all the reported spectrophotometric methods either need tedious extraction procedures, do not offer high sensitivity, use nonspecific reagent, or recommend the measurement of absorbance in the near UV region where interference most probably occurs that does not offer suitable linearity range. Result. A selective, sensitive, accurate, and precise, high performance liquid chromatographic method with UV detector analysis of Aceclofenac and Pregabalin was investigated. Good chromatographic separation was achieved using an ODS-BP hypersil C18 column 250 mm × 4.6 mm, i.d., 5 μm and a mobile phase consisting of 0.05 M phosphate buffer KH2PO4 pH 6.0 : methanol 60 : 40, v-v at a flow rate 1 mL-min. The ultraviolet detector was set at wavelength 218 nm. Retention time for Aceclofenac and Pregabalin was found to be 3.220 and 5.910 min, respectively. Rectilinear relationship with good regression coefficients 0.999 and 0.999 was found over the concentration ranges of 5–25 μg-mL and 3.75–18.75 μg-mL for ACF and PGB, respectively, with detection limits 0.64 and 0.35 μg-mL and quantitation limits 1.95 and 1.06 μg-mL. Conclusion. The mean percentage recoveries were in the range of 98.45–100.08 and 99.69–100.48 for ACF and PGB, respectively. The developed method was successfully applied to the analysis of the drugs in their commercial tablets.

Autor: Hitendrakumar D. Gelani, Payal P. Chauhan, and Samir K. Shah



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