Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism.Reportar como inadecuado

Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 IGM - Institut de génétique et microbiologie Orsay 2 Fonction, structure et inactivation d-ARN bactériens 3 Max Planck Institute for Infection Biology 4 MICALIS - MICrobiologie de l-ALImentation au Service de la Santé humaine 5 ICSN - Institut de Chimie des Substances Naturelles

Abstract : Using an experimental approach, we investigated the RNome of the pathogen Staphylococcus aureus to identify 30 small RNAs sRNAs including 14 that are newly confirmed. Among the latter, 10 are encoded in intergenic regions, three are generated by premature transcription termination associated with riboswitch activities, and one is expressed from the complementary strand of a transposase gene. The expression of four sRNAs increases during the transition from exponential to stationary phase. We focused our study on RsaE, an sRNA that is highly conserved in the bacillales order and is deleterious when over-expressed. We show that RsaE interacts in vitro with the 5- region of opp3A mRNA, encoding an ABC transporter component, to prevent formation of the ribosomal initiation complex. A previous report showed that RsaE targets opp3B which is co-transcribed with opp3A. Thus, our results identify an unusual case of riboregulation where the same sRNA controls an operon mRNA by targeting two of its cistrons. A combination of biocomputational and transcriptional analyses revealed a remarkably coordinated RsaE-dependent downregulation of numerous metabolic enzymes involved in the citrate cycle and the folate-dependent one-carbon metabolism. As we observed that RsaE accumulates transiently in late exponential growth, we propose that RsaE functions to ensure a coordinate downregulation of the central metabolism when carbon sources become scarce.

Autor: Chantal Bohn - Candice Rigoulay - Svetlana Chabelskaya - Cynthia Sharma - Antonin Marchais - Patricia Skorski - Elise Borezée-Du

Fuente: https://hal.archives-ouvertes.fr/


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