Antitumor effect and biological pathways of a recombinant adeno-associated virus as a human renal cell carcinoma suppressorReportar como inadecuado




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Tumor Biology

, Volume 35, Issue 11, pp 10993–11003

First Online: 05 August 2014Received: 18 April 2014Accepted: 23 July 2014

Abstract

The aims of this work are to study the antitumor effect of the adeno-associated virus on the xenografted tumors of chick embryo chorioallantoic membrane and predict potential genes and biological pathways which are associated with renal cell carcinoma. The adeno-associated virus NT4-TAT-6 × His-VHLbeta was constructed and identified. Then, chick embryos with xenografted tumor were divided into three groups and respectively inoculated with rAAV-NT4-TAT-6 × His-VHLbeta group A, empty virus group B, and phosphate-buffered saline group C, the control subject. Antitumor effect in each group was investigated by means of immunofluorescence observation. Genes interacted with von Hippel–Lindau were screened by Search Tool for the Retrieval of Interacting Genes-Proteins database, while pathway analysis were performed based on Kyoto Encyclopedia of Genes and Genomes. The growth of xenografted tumors inoculated with recombinant adeno-associated virus was slower than the control subjects. The tumor volumes of group A showed significant difference compared with group B and group C P < 0.05. Growth of xenografted tumors which administered with the recombinant adeno-associated virus was inhibited. Among the protein–protein interaction network, TCEB2, HIF1A, TCEB1, CUL2, RBX1, and PHF17 were hub genes which might be involved in the development of renal cell carcinoma. The most significant signaling pathway was renal cell carcinoma. In this paper, we constructed and identified the recombinant adeno-associated virus NT4-TAT-6 × His-VHLbeta and studied the antitumor effect of the adeno-associated virus on xenografted tumors of chicken embryo chorioallantoic membrane. In addition, genes in the protein–protein interaction network which are associated with renal cell carcinoma were revealed and the biological pathway of renal cell carcinoma was identified. Our results provide a gene-therapeutic agent for the treatment of human renal cell carcinoma.

KeywordsRenal cell carcinoma Recombinant adeno-associated virus Von Hippel–Lindau Protein–protein interaction network Pathway enrichment analysis Electronic supplementary materialThe online version of this article doi:10.1007-s13277-014-2393-z contains supplementary material, which is available to authorized users.

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Autor: Jie Chen - Xiyun Ruan - Shaomei Wang - Bin Zhang - Bo Liu - Zeqiang Sun - Qingyong Liu

Fuente: https://link.springer.com/article/10.1007/s13277-014-2393-z







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