Pf155-RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells.Reportar como inadecuado




Pf155-RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1 MIT - Massachusetts Institute of Technology 2 Korea Advanced Institute of Science and Technology 3 Immunologie moléculaire des parasites

Abstract : Proteins exported by Plasmodium falciparum to the red blood cell RBC membrane modify the structural properties of the parasitized RBC Pf-RBC. Although quasi-static single cell assays show reduced ring-stage Pf-RBCs deformability, the parameters influencing their microcirculatory behavior remain unexplored. Here, we study the dynamic properties of ring-stage Pf-RBCs and the role of the parasite protein Pf155-Ring-Infected Erythrocyte Surface Antigen RESA. Diffraction phase microscopy revealed RESA-driven decreased Pf-RBCs membrane fluctuations. Microfluidic experiments showed a RESA-dependent reduction in the Pf-RBCs transit velocity, which was potentiated at febrile temperature. In a microspheres filtration system, incubation at febrile temperature impaired traversal of RESA-expressing Pf-RBCs. These results show that RESA influences ring-stage Pf-RBCs microcirculation, an effect that is fever-enhanced. This is the first identification of a parasite factor influencing the dynamic circulation of young asexual Pf-RBCs in physiologically relevant conditions, offering novel possibilities for interventions to reduce parasite survival and pathogenesis in its human host.





Autor: Monica Diez-Silva - Yongkeun Park - Sha Huang - Hansen Bow - Odile Mercereau-Puijalon - Guillaume Deplaine - Catherine Lavazec -

Fuente: https://hal.archives-ouvertes.fr/



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