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Synchrotron microbeam irradiation induces neutrophil infiltration, thrombocyte attachment and selective vascular damage in vivo - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1 Institute of Anatomy 2 Institute of Pharmacology 3 EA RSRM 4 ESRF - European Synchrotron Radiation Facility

Abstract : Our goal was the visualizing the vascular damage and acute inflammatory response to micro-and minibeam irradiation in vivo. Microbeam MRT and minibeam radiation therapies MBRT are tumor treatment approaches of potential clinical relevance, both consisting of parallel X-ray beams and allowing the delivery of thousands of Grays within tumors. We compared the effects of microbeams 25– 100 μm wide and minibeams 200–800 μm wide on vasculature, inflammation and surrounding tissue changes during zebrafish caudal fin regeneration in vivo. Microbeam irradiation triggered an acute inflammatory response restricted to the regenerating tissue. Six hours post irradiation 6 hpi, it was infiltrated by neutrophils and fli1a + thrombocytes adhered to the cell wall locally in the beam path. The mature tissue was not affected by microbeam irradiation. In contrast, minibeam irradiation efficiently damaged the immature tissue at 6 hpi and damaged both the mature and immature tissue at 48 hpi. We demonstrate that vascular damage, inflammatory processes and cellular toxicity depend on the beam width and the stage of tissue maturation. Minibeam irradiation did not differentiate between mature and immature tissue. In contrast, all irradiation-induced effects of the microbeams were restricted to the rapidly growing immature tissue, indicating that microbeam irradiation could be a promising tumor treatment tool.





Autor: Daniel Brönnimann - Audrey Bouchet - Christoph Schneider - Marine Potez - Raphaël Serduc - Elke Bräuer-Krisch - Werner Graber

Fuente: https://hal.archives-ouvertes.fr/



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