Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell lineReportar como inadecuado

Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cancer Cell International

, 16:3

First Online: 09 February 2016Received: 27 June 2015Accepted: 03 February 2016


BackgroundBreast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hypericin on MCF-7 is uncertain. To investigate the cytotoxic effect of hypericin on MCF-7 cells, a human breast adenocarcinoma cell-line, that resistance to chemotherapy.

MethodsThe MCF-7 and fibroblast as normal cell line were treated with various concentrations of hypericin, and Cisplatin as a positive control for 24 and 48 h. Cytotoxicity activity was measured and confirmed by MTT assay and Trypan blue staining, respectively. In addition, Apoptosis were determined by Annexin V-Propidium Iodide assay. Immunocytochemistry ICC analysis for bcl2 and p53 proteins performed to further investigate different expression of these genes in different samples.

ResultsBoth cisplatin and the hypericin exhibited a dose-dependent cytotoxic effect in the MCF-7 cell line. Although the LD50 of the hypericin was significantly lower when compared to cispaltin 5 vs. 20 μg-ml, it continued to decrease the growth rate of the MCF-7 cells when tested at higher concentration than LD50. In contrast, cisplatine, at higher concentration than LD50, completely inhibited the growth of the MCF-7 in 48 h. Regarding Annexin V-Propidium results, treatment of MCF-7 cells with LD50 concentration of cisplatin and hypericin showed 60 and 52 % apoptosis in 24 h, respectively. ICC analysis for bcl2 and p53 also confirmed our results; in treated samples for the dose of LD50 in 24 and 48 h of cisplatin and hypercin, more cells expressed p53 guardian of cells in front of tumor formation-progression and less expressed bcl2 which has anti apoptotic activity compared to untreated samples.

ConclusionsConsidering that hypericin showed to be cytotoxic, it seems to be a chemopreventive agent and a good candidate for antineoplastic drug development.

KeywordsMCF-7 Breast Cancer Apoptosis Hypericum perforatum Hypericin Cytotoxic  Download fulltext PDF

Autor: Seyed Abbas Mirmalek - Mohammad Amin Azizi - Ehsan Jangholi - Soheila Yadollah-Damavandi - Mohammad Amin Javidi - Yekta P


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