Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growthReportar como inadecuado

Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growth - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 IGDR - Institut de Génétique et Développement de Rennes 2 Ligue Nationale Contre le Cancer - Paris 3 MRic - Centre de Microscopie de Rennes

Abstract : Intercellular bridges called -ring canals- RCs resulting from incomplete cytokinesis play an essential role in intercellular communication in somatic and germinal tissues. During Drosophila oogenesis, RCs connect the maturing oocyte to nurse cells supporting its growth. Despite numerous genetic screens aimed at identifying genes involved in RC biogenesis and maturation, how RCs anchor to the plasma membrane PM throughout development remains unexplained. In this study, we report that the clathrin adaptor protein 1 AP-1 complex, although dispensable for the biogenesis of RCs, is required for the maintenance of the anchorage of RCs to the PM to withstand the increased membrane tension associated with the exponential tissue growth at the onset of vitellogenesis. Here we unravel the mechanisms by which AP-1 enables the maintenance of RCs- anchoring to the PM during size expansion. We show that AP-1 regulates the localization of the intercellular adhesion molecule E-cadherin and that loss of AP-1 causes the disappearance of the E-cadherin-containing adhesive clusters surrounding the RCs. E-cadherin itself is shown to be required for the maintenance of the RCs- anchorage, a function previously unrecognized because of functional compensation by N-cadherin. Scanning block-face EM combined with transmission EM analyses reveals the presence of interdigitated, actin- and Moesin-positive, microvilli-like structures wrapping the RCs. Thus, by modulating E-cadherin trafficking, we show that the sustained E-cadherin-dependent adhesion organizes the microvilli meshwork and ensures the proper attachment of RCs to the PM, thereby counteracting the increasing membrane tension induced by exponential tissue growth.

Keywords : E-Cadherin trafficking ring canals membrane tension tissue growth

Autor: Nicolas Loyer - Irina Kolotuev - Mathieu Pinot - Roland Le Borgne -

Fuente: https://hal.archives-ouvertes.fr/


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