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The Scientific World Journal - Volume 2014 2014, Article ID 374276, 9 pages -

Research Article

Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China

Clinical Laboratory Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China

Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang 212013, China

Received 31 August 2013; Accepted 30 October 2013; Published 23 January 2014

Academic Editors: R. Tofalo and J. Yoon

Copyright © 2014 Min Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Salmonella enterica serovar Typhi S. Typhi is a human-specific pathogen that causes typhoid fever. In this study, we constructed ygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli E. coli, though the sequence of ygaE is totally different from gabC formerly ygaE in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi.

Autor: Min Wang, Ping Feng, Xun Chen, Haifang Zhang, Bin Ni, Xiaofang Xie, and Hong Du

Fuente: https://www.hindawi.com/


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