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Journal of Nanomaterials - Volume 2015 2015, Article ID 371529, 8 pages -

Research Article

Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia

Department of Veterinary Services, Ministry of Animal Health and Fisheries Development, Usman Faruk Secretariat, PMB 2109, Sokoto 840221, Sokoto State, Nigeria

Institute of Bioscience, Universiti Putra Malaysia UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia

Received 3 April 2015; Revised 25 May 2015; Accepted 28 May 2015

Academic Editor: Subrata Kundu

Copyright © 2015 Faruku Bande et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nonviral delivery system receives attention over the last decade. Chitosan CS is a cationic polymer whereas saponin SP is classified as glycoside. In this study, a spherically-shaped CS-SP nanoparticle was synthesized and characterized. The ability of the nanoparticle to protect DNA from enzymatic degradation, its thermostability and cytotoxicity were evaluated. The particle size was found below 100 nm as determined by Zetasizer, transmission electron microscopy TEM, and field scanning electron microscopy FSEM results. The surface charge ranges from 43.7 mV to 38.5 mV before and after encapsulation with DNA plasmid, respectively. In terms of thermostability, Thermal Gravimetric Analysis TGA and Differential Scanning Calorimetry DSC revealed that CS-SP nanoparticle had a melting temperature of 110°C, with rapid decomposition occurring at 120°C. Encapsulation of DNA with the synthesized nanoparticle was evidenced by changes in the FTIR spectra including characteristic peaks at 3267.39 and 1635.58 cm

, wavenumbers. Additional peak was also observed at 1169.7 cm

following encapsulation. Electrophoretic mobility showed that CS-SP nanoparticle protected plasmid DNA from enzymatic degradation, while cell viability assays confirmed that the synthesized nanoparticle exhibited low cytotoxicity at different concentrations in avian cells. Taken together these, CS-SP nanoparticle showed potentials for applications as a DNA delivery system.





Autor: Faruku Bande, Siti Suri Arshad, Mohd Hair Bejo, Shafiu Abdullahi Kamba, and Abdul Rahman Omar

Fuente: https://www.hindawi.com/



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