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Cancer Cell International

, 17:17

First Online: 02 February 2017Received: 10 October 2016Accepted: 25 December 2016

Abstract

BackgroundRecently, miR-22 is identified as a tumor-suppressing microRNA in many human cancers. CD147 is a novel cancer-associated biomarker that plays an important role in the invasion and metastasis of malignant tumor. However, the involvement of miR-22 in CD147 regulation and hepatocellular carcinoma HCC progression and metastasis has not been investigated.

MethodsWe measured miR-22 expression level in 34 paired of HCC and matched normal tissues, HCC cell lines by real-time quantitative RT-PCR. Invasion assay, MTT proliferation assay and wound-healing assay were performed to test the invasion and proliferation of HCC cell after overexpression of miR-22. The effect of miR-22 on HCC in vivo was validated by murine xenograft model. The relationship of miR-22 and its target gene CD147 was also investigated.

ResultsWe found that the expression of miR-22 in HCC tissues and cell lines were much lower than that in normal control, respectively. The expression of miR-22 was inversely correlated with HCC metastatic ability. Moreover, overexpression of miR-22 could significantly inhibit the HCC cell proliferation, migration and invasion in vitro and decrease HCC tumor growth in vivo. Finally, we found that miR-22 interacted with CD147 and decreased its expression, via a specific target site within the CD147 3′UTR by luciferase reporter assay. The expression of CD147 was inversely correlated with miR-22 expression in HCC tissues.

ConclusionOur results suggested that miR-22 was downexpressed in HCC and inhibited HCC cell proliferation, migration and invasion through downregulating cancer-associated gene CD147 which may provide a new bio-target for HCC therapy.

KeywordsmiR-22 Hepatocellular carcinoma Invasion Migration CD147 AbbreviationsHCChepatocellular carcinoma

ANTadjacent non-tumor

RT-PCRreverse transcription polymerase chain reaction

MTT3-4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide

RNAribonucleic acid

mRNAmessenger RNA

GAPDHglyceraldehyde-3-phosphate dehydrogenase

Ling-Juan Luo and Li-Ping Zhang contributed equally to this work

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Autor: Ling-Juan Luo - Li-Ping Zhang - Chun-Yan Duan - Bei Wang - Na-Na He - Patima Abulimiti - Yan Lin

Fuente: https://link.springer.com/article/10.1186/s12935-016-0380-8



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